Scientist I
Location: Waltham, MA
Duration: Contract till Sept 2025 with possibility to extend
Scientist, Liver gene therapy mechanism of action, Genomic Medicine Unit
Basic Qualifications
• Demonstrated expertise with standard aseptic cell culture technique, including experience culturing various primary cells. Experience with hepatocytes primary cell culture is highly appreciated. Experience with hepatocytes and/or liver non-parenchymal cells isolation is a plus.
• Broad experience with cell-based functional assays, including transfection, cytotoxicity, cytokine release, and proliferation assays. Experience with co-culture in-vitro or MPS is a plus.
• Proficiency across cellular, biochemical, and molecular techniques including protein detection (ELISA, MSD, western blot), gene profiling (RNA/DNA extraction, qPCR) and cell imaging. Experience with high content microscopy is a plus.
• Broad scientific knowledge in liver biology and non-viral delivery and molecular biology.
• Self-motivated with strong time management and organizational skills
• Flexibility to rapidly changing priorities and deadlines
• Experience in preparing high quality documents including technical reports, presentations, SOPs, and experimental protocols.
• Highly collaborative with demonstrated ability to work in a matrixed team environment.
• Excellent oral and written communication skills
Key Responsibilities:
• Maintain cultures of immortalized tumor cell lines, hepatic primary cell types (e.g. hepatocytes, LSEC and KC).
• Perform hepatocytes mouse isolation from key disease models.
• Optimize, design, execute, and interpret in vitro cell-based assays to characterize diverse mechanisms of gene therapies (e.g. ELISA, MSD, high content imaging)
• Perform fit-for-purpose in vitro and ex vivo assays to assess phenotypic and functional read-outs to assess drug candidate toxicity and efficacy.
• Prepare experimental protocols, analyze experimental data, and present study results in team and department meetings.
• Document experimental methods and findings in electronic laboratory notebook
POSITION OBJECTIVES
Conduct and interpret cell-based in vitro assays to characterize mechanism of action related to non-viral gene therapy with focus on the hepatic environment:
• Immunomodulation
• Cargo cell localization
• Support non-viral (LNP) oligo therapy screenings e.g. endosomal scape assessment
Thanks & Regards
Aamir siddiqui
Lead Recruiter
O 908-293-6164
E aamir.siddiqui@aequor.com
W https://www.aequor.com/
